Detection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining
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Authors
Lariviere, Wells B.
Han, Xiaorui
Oshima, Kaori
McMurtry, Sarah A.
Linhardt, Robert J.
Schmidt, Eric P.
Issue Date
2021-02-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Sulfated glycosaminoglycans (GAGs) such as heparan sulfate (HS) and chondroitin sulfate (CS) are ubiquitous in living organisms and play a critical role in a variety of basic biological structures and processes. As polymers, GAGs exist as a polydisperse mixture containing polysaccharide chains that can range from 4000 Da to well over 40,000 Da. Within these chains exists domains of sulfation, conferring a pattern of negative charge that facilitates interaction with positively charged residues of cognate protein ligands. Sulfated domains of GAGs must be of sufficient length to allow for these electrostatic interactions. To understand the function of GAGs in biological tissues, the investigator must be able to isolate, purify, and measure the size of GAGs. This report describes a practical and versatile polyacrylamide gel electrophoresis-based technique that can be leveraged to resolve relatively small differences in size between GAGs isolated from a variety of biological tissue types.
Description
Journal of Visualized Experiments, 168, e62319
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Detection of glycosaminoglycans by polyacrylamide gel electrophoresis and silver staining, W. B. LaRiviere, X. Han, K. Oshima, S. A. McMurtry, R. J. Linhardt, E. P. Schmidt, Journal of Visualized Experiments, 168, e62319, 2021.
Publisher
Jove
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
1940087X