Engineering recombinant microbial communities for cellulose degradation and utilization

Kalbarczyk, Karolina Zbigniew
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Collins, Cynthia H.
Koffas, Mattheos A. G.
Tessier, Peter M.
Barquera, Blanca L.
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Chemical engineering
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Cellulase activity against amorphous cellulose was confirmed, and the most active SP constructs were identified as EGI1 with the LipA SP and Cel9AT with the YngK SP. Both strains were optimized for cellulase expression, and activity of the enzymes was characterized individually and in tandem to demonstrate synergistic cellulolytic activity in coculture. The secreted cellulases were reacted with amorphous cellulose under a range of different conditions, and the resulting glucose supported the growth of the second target module, an E. coli strain to be used for production of violacein, an antimicrobial pharmaceutical product with anti-tumor properties. A series of studies investigated the compatibility of the two modules and the cellulose degradation reaction. The construction of a two-module system for efficient cellulose degradation and utilization demonstrated the value of the modular system design, to allow bacterial strains to be interchanged or linked with other engineered bacterial strains for the efficient production of high value molecules.
August 2018
School of Engineering
Dept. of Chemical and Biological Engineering
Rensselaer Polytechnic Institute, Troy, NY
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