Glycosaminoglycan characterization by electrospray ionization mass spectrometry including Fourier transform mass spectrometry

Loading...
Thumbnail Image
Authors
Laremore, Tatiana N.
Leach, Franklin E.
Solakyildirim, Kemal
Amster, I. Jonathan
Linhardt, Robert J.
Issue Date
2010-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Research Projects
Organizational Units
Journal Issue
Alternative Title
Abstract
Electrospray ionization mass spectrometry (ESI MS) is a versatile analytical technique in glycomics of glycosaminoglycans (GAGs). Combined with enzymology, ESI MS is used for assessing changes in disaccharide composition of GAGs biosynthesized under different environmental or physiological conditions. ESI coupled with high-resolution mass analyzers such as a Fourier transform mass spectrometer (FTMS) permits accurate mass measurement of large oligosaccharides and intact GAGs as well as structural characterization of GAG oligosaccharides using information-rich fragmentation methods such as electron detachment dissociation. The first part of this chapter describes methods for disaccharide compositional profiling using ESI MS and the second part is dedicated to FTMS and tandem MS methods of GAG compositional and structural analysis.
Description
Methods in Enzymology, 478, 79-108
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Glycosaminoglycan characterization by electrospray ionization mass spectrometry including Fourier transform mass spectrometry, T. N. Laremore, F. E. Leach III, K. Solakyildirim, I. J. Amster, R. J. Linhardt, Methods in Enzymology, 478, 79-108, 2010.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
766879
EISSN