Ultraperformance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

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Authors
Yang, B.
Weyers, A.
Baik, J.Y.
Sterner, E.
Sharfstein, S.
Mousa, S.A.
Zhang, F.
Dordick, J.S.
Linhardt, Robert J.
Issue Date
2011
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Article
Language
ENG
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
A high resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultraperformance liquid chromatography in a reverse-phase, ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues and biological fluids, as it provides high sensitivity without being subject to interference from proteins, peptides and other sample impurities.
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Analytical Biochemistry, 415, 59–66
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Full Citation
Ultraperformance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis, B. Yang, A. Weyers, J. Y. Baik, E. Sterner, S. Sharfstein, S. A. Mousa, F. Zhang, J. S. Dordick, R. J. Linhardt, Analytical Biochemistry, 415, 59–66, 2011.
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Elsevier
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