One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
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Authors
Fu, Li
Linhardt, Robert J.
Issue Date
2022-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → 4)-β-D-N-acetylglucosamine (1→, corresponds to the precursor backbone in the Golgi-based biosynthesis of heparin. Anticoagulant heparin is prepared in a one-pot synthesis using a chemically prepared derivative of heparosan called N-sulfoheparosan (NSH), recombinant Golgi enzymes expressed in E. coli, and the 3-phosphoadenosine-5-phosphosulfate (PAPS) cofactor.
Description
Methods in Molecular Biology, 2303, 3-11
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan, L. Fu, R. J. Linhardt, Methods in Molecular Biology, 2303, 3-11, 2022.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
19406029
10643745
10643745