One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan

Fu, Li
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan, L. Fu, R. J. Linhardt, Methods in Molecular Biology, 2303, 3-11, 2022.
Heparin, a glycosaminoglycan-based anticoagulant drug, is prepared as an extract of animal tissues. Heparosan, an Escherichia coli (E. coli) K5 capsular polysaccharide with the structure →4)-β-D-glucuronic acid (1 → 4)-β-D-N-acetylglucosamine (1→, corresponds to the precursor backbone in the Golgi-based biosynthesis of heparin. Anticoagulant heparin is prepared in a one-pot synthesis using a chemically prepared derivative of heparosan called N-sulfoheparosan (NSH), recombinant Golgi enzymes expressed in E. coli, and the 3-phosphoadenosine-5-phosphosulfate (PAPS) cofactor.
Methods in Molecular Biology, 2303, 3-11
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Rensselaer Polytechnic Institute, Troy, NY
Methods in Molecular Biology