Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells

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Authors
Datta, Payel
Yang, Bo
Linhardt, Robert J.
Sharfstein, Susan T.
Issue Date
2015-03-18
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Sodium butyrate, a histone deacetylase inhibitor, has been used to improve transgene expression in Chinese hamster ovary (CHO) cells. The current study explores the impact of butyrate treatment on heparan sulfate (HS) biosynthesis and structural composition in a recombinant CHO-S cell line expressing enzymes in the heparin (HP)/(HS) biosynthetic pathway (Dual-10 stably expressing NDST2 and HS3st1). Flow cytometric analysis showed that antithrombin binding was increased in Dual-10 cells and basic fibroblast growth factor binding was decreased in response to sodium butyrate treatment. The results were in agreement with the AMAC-LCMS (2-aminoacridine-tagged HS/HP analysis by liquid chromatography mass spectrometry) data that showed that there was an increase in heparan sulfate tri-sulfated disaccharides and a decrease in N-sulfated disaccharides in the butyrate-treated cells. However, we could not detect any changes in the chondroitin sulfate pathway in Dual-10 cells treated with butyrate. The current study is the first to report the effect of butyrate on glycosaminoglycan profiles.
Description
Cytotechnology, 67, 223–235
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Full Citation
Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells, P. Datta, B. Yang, R. J. Linhardt, S.T. Sharfstein, Cytotechnology, 67, 223–235, 2015.
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ISSN
15730778
9209069
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