Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate

Authors
Chi, Lianli
Munoz, Eva M.
Choi, Hyung Seok
Ha, Young Wan
Kim, Yeong Shik
Toida, Toshihiko
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2006-05-22
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Full Citation
Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate, L. Chi, E. M. Munoz, H. S. Choi, Y. W. Ha, Y. S. Kim, T. Toida, R. J. Linhardt, Carbohydrate Research, 341 864–869, 2006.
Abstract
The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate, -->4)-alpha-d-GlcN(p)Ac-(1-->4)-alpha-l-IdoA(p)2S-(1-->, terminated by 4-linked alpha-d-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end.
Description
Carbohydrate Research, 341, 864–869
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Research
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI