Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate
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Authors
Chi, Lianli
Munoz, Eva M.
Choi, Hyung Seok
Ha, Young Wan
Kim, Yeong Shik
Toida, Toshihiko
Linhardt, Robert J.
Issue Date
2006-05-22
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate, -->4)-alpha-d-GlcN(p)Ac-(1-->4)-alpha-l-IdoA(p)2S-(1-->, terminated by 4-linked alpha-d-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end.
Description
Carbohydrate Research, 341, 864–869
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Preparation and Structural Determination of Large Oligosaccharides Derived from Acharan Sulfate, L. Chi, E. M. Munoz, H. S. Choi, Y. W. Ha, Y. S. Kim, T. Toida, R. J. Linhardt, Carbohydrate Research, 341 864–869, 2006.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
86215