Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization
No Thumbnail Available
Authors
Zhang, Anqiang
Deng, Jiaying
Liu, Xiaoqing
He, Pengfei
He, Liang
Zhang, Fuming
Linhardt, Robert J.
Sun, Peilong
Issue Date
2018-07-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Agaricus blazei Murill is an edible and medicinal mushroom favored in many countries, by virtue of both its delicious taste and its potential health benefits such as its purported anticancer activity. A neutral α-glucan (ABM40-1) with a carbohydrate content of 96% was purified from the high-speed shearing homogenization extracts of A. Blazei Murill by ethanol precipitation and column chromatography. Methylation analysis along with nuclear magnetic resonance spectroscopy revealed that ABM40-1 was an α-(1 → 4)-d-glucopyranan with O-6 position occasionally occupied with α-Glcp-(1 → or α-Glcp-(1 → 6)-β-Glcp-(1 → side chains. A weight-average molecular weight of 7.34 × 106 Da was determined for ABM40-1 and its chain in solution was revealed as a compact sphere by size exclusion chromatography (SEC) coupled with a laser light scattering. This spherical conformation was also further confirmed by Congo red test and using atom force microscopy. These results suggest it would be worthwhile to further study the potential bioactivities of ABM40-1.
Description
International Journal of Biological Macromolecules, 113, 558–564
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization, A. Zhang, J. Deng, X. Liu, P. He, L. He, F. Zhang, R.J. Linhardt, P. Sun, International Journal of Biological Macromolecules, 113, 558–564, 2018.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
18790003
1418130
1418130