Biochemical and structural characterization of intein inhibitors for use as anti-mycobacterial therapeutics

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176923_Chan_rpi_0185N_10770.pdf (2.56 MB)
Authors
Chan, Hon
Issue Date
2015-12
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Electronic thesis
Thesis
Language
ENG
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Chemical engineering
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Abstract
The human pathogen Mycobacterium tuberculosis harbor inteins, self-splicing protein elements, in critical dnaB, recA and sufB genes. Inteins render the host protein non-functional until splicing occurs, thus inhibition of splicing provides a novel target for design of anti-mycobacterial compounds. The splicing inhibition of a minimized RecA intein (Mini-RecAi) was investigated, firstly through a screening a series of candidate molecules. Secondly the binding of cisplatin, a FDA approved chemotherapeutic and potent inhibitor of Mini-RecAi, was characterized using mass spectrometry, nuclear magnetic resonance and crystallographic methods.
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December 2015
School of Engineering
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Rensselaer Polytechnic Institute, Troy, NY
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https://hdl.handle.net/20.500.13015/1587
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RPI Theses Online (Complete)