Effect of Fully Sulfated Glycosaminoglycans on Pulmonary ArterySmooth Muscle Cell Proliferation
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Authors
Garg, H.G.
Joseph, P.A.M.
Thompson, T.
Hales, C.A.
Toida, T.
Imanari, T.
Capila, I.
Linhardt, Robert J.
Issue Date
1999
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Fully sulfated heparin and other glycosaminoglycans, namely heparan, chondroitin, and dermatan sulfates, and hyaluronan have been prepared by using sulfur trioxide under mild chemical conditions. All these derivatives were assayed for antiproliferative activity on cultured bovine pulmonary artery smooth muscle cells (BPASMCs). No appreciable difference was found between heparin and fully sulfated heparin. Chondroitin and dermatan sulfates actually stimulated BPASMCs growth but full sulfonation made them strongly antiproliferative. Native hyaluronan was not antiproliferative but became strongly so after sulfonation. Neither acharan sulfate nor N-sulfoacharan sulfate had any antiproliferative activity. This suggests that O-sulfonation of the polysaccharide is critical for antiproliferative activity, whereas N-sulfonation of glucosamine residues is not.
Description
Archivesin Biochemistry and Biophysics, 371, 228-233
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Effect of Fully Sulfated Glycosaminoglycans on Pulmonary ArterySmooth Muscle Cell Proliferation, H. G. Garg, P.A.M. Joseph, T. Thompson,C.A. Hales, T. Toida, T. Imanari, I. Capila, R.J. Linhardt, Archivesin Biochemistry and Biophysics, 371, 228-233, 1999.