Flexible electrospun cellulose fibers as an affinity packing material for the separation of bovine serum albumin
No Thumbnail Available
Authors
Miyauchi, M.
Miao, J.
Simmons, T.J.
Dordick, J.S.
Linhardt, Robert J.
Issue Date
2011
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Flexible, well-dispersed and continuous 100-nm diameter cellulose fibers were prepared from an ionic liquid solvent by a novel dry-jet wet-electrospinning process. The ribbon fibers formed were chemically activated and an affinity dye, cibacron blue (CB) was immobilized at 0.22 g CB/g dry fibers loading to the surface of these fibers. The resulting affinity matrix was packed into a chromatography column and the adsorption, desorption, and specificity of this matrix for bovine serum albumin (BSA) was studied. These electrospun fibers had a BSA binding capacity of 230 mg/g, nearly twice that of CB-immobilized 100-um beads and over ten-fold higher capacity that CB-immobilized cellulose fibers prepared by a conventional electrospinning process. The results of this work suggest that chromatography supports of flexible, well-dispersed, continuous nanofibers may offer advantages over conventional supports in affinity separations.
Description
Journal of Chromatography and Separation Techniques, 2,1-4
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Flexible electrospun cellulose fibers as an affinity packing material for the separation of bovine serum albumin, M. Miyauchi, J. Miao, T. J. Simmons, J. S. Dordick, R.J. Linhardt, Journal of Chromatography and Separation Techniques, 2,1-4, 2011.