Poly(ethylene glycol)-Based Biosensor Chip to Study Heparin Protein Interactions

Muñoz, E.M.
Yu, H.
Hallock, J.
Edens, R.E.
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Poly(ethylene glycol)-Based Biosensor Chip to Study Heparin Protein Interactions, E.M. Muñoz, H. Yu, J. Hallock, R.E. Edens, R.J. Linhardt, 343, 176-178, 2005. Analytical Biochemistry, 343, 176-178, 2005.
Heparin’s role in biological processes is commonly mediated by its interaction with proteins [1]. During the past decade, our research group has relied on surface plasmon resonance (SPR)1 to monitor heparin–protein interactions [2–8]. Heparin is typically immobilized on the chip, and protein flows over the heparinized surface. Unfortunately, nonspecific interaction between analyte and the chip surface is frequently encountered. Although physical adsorption of the protein can be minimized using surfaces coated with hydrophilic polymers such as dextran, we have recently encountered a number of heparin-binding proteins that interact with dextran. Poly(ethylene glycol) (PEG)-based sensor chips might represent an alternative to the dextran-based sensor chips, for the study of heparin–protein interactions, because surfaces grafted with PEG typically show reduced protein adsorption [9]. In the current work, we describe the application of a PEG-based SPR sensor chip to acquire kinetic and affinity data on the interaction of complement protein factor P with heparin, an that could not be studied with commercially available sensor chips.
Analytical Biochemistry, 343, 176-178
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