Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery
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Authors
Olmsted, Zachary T.
Stigliano, Cinzia
Badri, Abinaya
Zhang, Fuming
Williams, Asher
Koffas, Mattheos A.G.
Xie, Yubing
Linhardt, Robert J.
Cibelli, Jose
Horner, Philip J.
Issue Date
2020-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Cell therapy for the injured spinal cord will rely on combined advances in human stem cell technologies and delivery strategies. Here we encapsulate homotypic spinal cord neural stem cells (scNSCs) in an alginate-based neural ribbon delivery platform. We perform a comprehensive in vitro analysis and qualitatively demonstrate graft survival and injury site retention using a rat C4 hemi-contusion model. Pre-configured neural ribbons are transport-stable modules that enable site-ready injection, and can support scNSC survival and retention in vivo. Neural ribbons offer multifunctionality in vitro including co-encapsulation of the injury site extracellular matrix modifier chondroitinase ABC (chABC), tested here in glial scar models, and ability of cervically-patterned scNSCs to differentiate within neural ribbons and project axons for integration with 3-D external matrices. This is the first extensive in vitro characterization of neural ribbon technology, and constitutes a plausible method for reproducible delivery, placement, and retention of viable neural cells in vivo.
Description
Scientific Reports 10, 12939
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery, Z. T. Olmsted, C. Stigliano, A. Badri, F. Zhang, A. Williams, M. A. G. Koffas, Y. Xie, R. J. Linhardt, J. Cibelli, P. J. Horner, J. L. Paluh, Scientific Reports 10, 12939, 2020
Publisher
Nature
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
20452322