Separation of Hydroxyl-Protected Heparin Derived Disaccharides using Reversed-Phase High Performance Liquid Chromatography
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Authors
Kerns, R.J.
Linhardt, Robert J.
Issue Date
1995-06-30
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A simple and efficient method for the separation of hydrophobic derivatives of glycosaminoglycan-derived disaccharides is described. Hydroxyl-protected derivatives of a trisulfated disaccharide, prepared from heparin using heparin lyase, were separated by reversed-phase high-performance liquid chromatography. These disaccharide derivatives differed by the number, position, and stereochemistry of acetyl and pivaloyl groups. Separation was achieved on a C18 column using a reversed gradient of ammonium sulfate in water. This method has application in the purification of disaccharide derivatives being used as chiral synthons in the preparation of higher oligosaccharides.
Description
Journal of Chromatography A, 705, 369-373
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Separation of Hydroxyl-Protected Heparin Derived Disaccharides using Reversed-Phase High Performance Liquid Chromatography, R. J. Kerns, R. J. Linhardt, Journal of Chromatography A, 705, 369-373, 1995.
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Journal
Volume
Issue
PubMed ID
DOI
ISSN
219673