Localization and Characterization of Acharan Sulfate in the Body of the Giant African Snail Achatina fulica
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Authors
Jeong, Jia
Toida, Toshihiko
Muneta, Yuki
Kosiishi, Ichiro
Imanari, Toshio
Linhardt, Robert J.
Choi, Hyung Seok
Wu, Song Ji
Kim, Yeong Shik
Issue Date
2001-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Acharan sulfate is a glycosaminoglycan (GAG), having the structure -->4)-2-acetamido-2-deoxy-alpha-D-glucopyranose(1-->4)-2-sulfo-alpha-L-idopyranosyluronic acid (1-->, isolated from the body of the giant African snail Achatina fulica. This GAG represents 3-5% of the dry weight of this snail's soft body tissues. Frozen sections and polyester wax sections of the snail's body were stained by Alcian blue-periodic acid-Schiff's reagent (PAS) to localize acharan sulfate. Alcian blue staining indicated that GAG was mainly secreted into the outer surface of the body from internal granules. A highly mucous material was collected and treated and the acharan sulfate was recovered by ethanol and cetyl pyridinium chloride precipitation. Crude acharan sulfate was purified by DEAE-Sephacel ion-exchange chromatography. Depolymerization of intact mucus and purified acharan sulfate fractions by heparin lyase II (heparitinase I) from Flavobacterium heparinum produced an unsaturated disaccharide as a major product, establishing the repeating unit of acharan sulfate. These results demonstrate that mucus in the granule and secreted to the outside of the body is composed entirely of acharan sulfate.
Description
Comparative Biochemistry and Physiology (Part B), 130, 513-519
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Localization and Characterization of Acharan Sulfate in the Body of the Giant African Snail Achatina fulica, J. Jeong, T. Toida, I. Kosiishi, T. Imanari, R.J. Linhardt, S.J. Wu, Y.S. Kim, Comparative Biochemistry and Physiology (Part B), 130, 513-519, 2001.
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ISSN
10964959