A novel structural fucosylated chondroitin sulfate from Holothuria mexicana and its effects on growth factors binding and anticoagulation
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Authors
Li, Qinying
Cai, Chao
Chang, Yaoguang
Zhang, Fuming
Linhardt, Robert J.
Xue, Changhu
Li, Guoyun
Yu, Guangli
Issue Date
2018-02-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated from sea cucumber Holothuria Mexicana (FCShm) was characterized by NMR spectra and HILIC-FTMS, which demonstrated four types of branches in FCShm. Among these, two branches were α-L-Fuc-2S4S (where Fuc is fucose and S is sulfo) and α-L-Fuc-4S linked to O-3 of glucuronic acid residues, while others were identified as α-L-Fuc-4S and α-L-Fuc-3S4S attached to O-6 of N-acetylgalactosamine residue. Furthermore, the fucosyl branches were α-1,3-linked with different degree of polymerization from 1 to 5. FCShm exhibited high affinity to fibroblast growth factor 1 and 2, growth factors involved in neovascularization. Moreover, FCShm displayed intrinsic anticoagulant activity and inhibited thrombin and factor Xa activation by antithrombin III. Our results proposed a novel structural FCS and demonstrated its favorable application prospects in anti-angiogenesis and anticoagulation.
Description
Carbohydrate Polymers, 181, 1160-1168
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
A novel structural fucosylated chondroitin sulfate from Holothuria mexicana and its effects on growth factors binding and anticoagulation, Q. Li, C. Cai, Y. Chang, F. Zhang, R. J. Linhardt, C. Xue, G. Li, G. Yu, Carbohydrate Polymers, 181, 1160-1168, 2018
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ISSN
1448617