Sensitive Cells: Enabling Tools for Static and Dynamic Control of Microbial Metabolic Pathways

Cress, Brady F.
Trantas, Emmanouil A.
Ververidis, Filippos
Linhardt, Robert J.
Koffas, Mattheos A.G.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Sensitive Cells: Enabling Tools for Static and Dynamic Control of Microbial Metabolic Pathways, B. F. Cress, E. A. Trantas, F. Ververidis, R. J. Linhardt, M. Koffas, Current Opinion in Biotechnology, 36, 205-214, 2015.
Natural metabolic pathways are dynamically regulated at the transcriptional, translational, and protein levels. Despite this, traditional pathway engineering has relied on static control strategies to engender changes in metabolism, most likely due to ease of implementation and perceived predictability of design outcome. Increasingly in recent years, however, metabolic engineers have drawn inspiration from natural systems and have begun to harness dynamically controlled regulatory machinery to improve design of engineered microorganisms for production of specialty and commodity chemicals. Here, we review recent enabling technologies for engineering static control over pathway expression levels, and we discuss state-of-the-art dynamic control strategies that have yielded improved outcomes in the field of microbial metabolic engineering. Furthermore, we emphasize design of a novel class of genetically encoded controllers that will facilitate automatic, transient tuning of synthetic and endogenous pathways.
Current Opinion in Biotechnology, 36, 205-214
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Current Opinion in Biotechnology