Sensitive Cells: Enabling Tools for Static and Dynamic Control of Microbial Metabolic Pathways
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Authors
Cress, Brady F.
Trantas, Emmanouil A.
Ververidis, Filippos
Linhardt, Robert J.
Koffas, Mattheos A.G.
Issue Date
2015-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Natural metabolic pathways are dynamically regulated at the transcriptional, translational, and protein levels. Despite this, traditional pathway engineering has relied on static control strategies to engender changes in metabolism, most likely due to ease of implementation and perceived predictability of design outcome. Increasingly in recent years, however, metabolic engineers have drawn inspiration from natural systems and have begun to harness dynamically controlled regulatory machinery to improve design of engineered microorganisms for production of specialty and commodity chemicals. Here, we review recent enabling technologies for engineering static control over pathway expression levels, and we discuss state-of-the-art dynamic control strategies that have yielded improved outcomes in the field of microbial metabolic engineering. Furthermore, we emphasize design of a novel class of genetically encoded controllers that will facilitate automatic, transient tuning of synthetic and endogenous pathways.
Description
Current Opinion in Biotechnology, 36, 205-214
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Sensitive Cells: Enabling Tools for Static and Dynamic Control of Microbial Metabolic Pathways, B. F. Cress, E. A. Trantas, F. Ververidis, R. J. Linhardt, M. Koffas, Current Opinion in Biotechnology, 36, 205-214, 2015.
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Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
18790429
9581669
9581669