Developing millimeter-scale indentation to probe local cellular environments
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Authors
Dass, Rachel Elizabeth
Issue Date
2018-12
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biomedical engineering
Alternative Title
Abstract
It was found that agarose indentation elastic modulus increases with concentration (w/v) and that stress-relaxation properties generally remain constant across concentrations. Solvent-type and aging of agarose over a two week period do not change indentation elastic modulus. In addition, this indentation technique is capable of measuring the mechanical properties of other in vitro models and tissue materials. Fiber-reinforced hydrogels, a novel hybrid fiber-gel in vitro ECM model, were tested as an additional in vitro material. Explant tissue, rat mammary glands, were also measured for comparison with in vitro models. Overall, this milli-scale indentation provides a versatile technique to assess elastic and viscoelastic material properties for improved design and development of in vitro models for a wide range of tissue engineering applications.
Description
December 2018
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY