Analysis of glycosaminoglycans in stem cell glycomics
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Authors
Li, Boyangzi
Liu, Haiying
Zhang, Zhenqing
Stansfield, Hope E.
Dordick, Jonathan S.
Linhardt, Robert J.
Issue Date
2011-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.
Description
in Embryonic Stem Cell Therapy for Osteodegenerative Diseases. Nicole zur Nieden, Ed., Humana Press, Methods in Molecular Biology 690, 285-300
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Analysis of glycosaminoglycans in stem cell glycomics, B. Li, H. Liu, Z. Zhang, H. Stansfield, J.S. Dordick, R.J. Linhardt, in Embryonic Stem Cell Therapy for Osteodegenerative Diseases. Nicole zur Nieden, Ed., Humana Press, Methods in Molecular Biology 690, 285-300, 2011.
Publisher
Springer
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10643745