Analysis of glycosaminoglycans in stem cell glycomics

Authors
Li, Boyangzi
Liu, Haiying
Zhang, Zhenqing
Stansfield, Hope E.
Dordick, Jonathan S.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2011-12-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Analysis of glycosaminoglycans in stem cell glycomics, B. Li, H. Liu, Z. Zhang, H. Stansfield, J.S. Dordick, R.J. Linhardt, in Embryonic Stem Cell Therapy for Osteodegenerative Diseases. Nicole zur Nieden, Ed., Humana Press, Methods in Molecular Biology 690, 285-300, 2011.
Abstract
Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.
Description
in Embryonic Stem Cell Therapy for Osteodegenerative Diseases. Nicole zur Nieden, Ed., Humana Press, Methods in Molecular Biology 690, 285-300
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Springer
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Methods in Molecular Biology
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI