Production of deuterated cyanidin 3-O-glucoside from recombinant Escherichia coli
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Authors
Gupta, Mamta
Zha, Jian
Zhang, Xing
Jung, Gyoo Yeol
Linhardt, Robert J.
Koffas, Mattheos A.G.
Issue Date
2018-09-30
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Anthocyanins are plant secondary metabolites that, despite their chemical instability, have found many applications as natural food colorants. They are also known for their beneficial health effects because of their antioxidant and anticancer properties. More stable versions of these molecules, particularly at neutral pH conditions, are required to study the anthocyanin pharmacokinetic properties and obtain effective therapeutic results. In the present report, a cost-effective technique was developed to prepare the deuterated anthocyanin using recombinant Escherichia coli as a production host and deuterated glycerol and D2O in the culture media. This approach resulted in the formation of endogenous deuterated uridine 5′-diphosphate-glucose that was further incorporated by the recombinant anthocyanin pathway, resulting in the formation of deuterated cyanidin 3-O-glucoside (C3G). The deuterium exchange of O–D and C–D were studied by liquid chromatography (LC)–mass spectrometry and NMR analysis. The labeled C3G, purified by high-performance LC showed a stable nature at pH 7.0 as compared to nondeuterated C3G.
Description
ACS Omega, 3, 11643−11648
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Production of deuterated cyanidin 3-O-glucoside from recombinant Escherichia coli M. Gupta, J. Zha, X. Zhang, G.-Y. Jung, R. J. Linhardt, M. Koffas, ACS Omega, 3, 11643−11648, 2018.
Publisher
American Chemical Society (ACS)
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
24701343