Inhibition of glucuronomannan hexamer on the proliferation of lung cancer through binding with immunoglobulin G

Jin, Weihua
He, Xinyue
Zhu, Jiachen
Fang, Qiufu
Wei, Bin
Sun, Jiadong
Zhang, Wenjing
Zhang, Zhongshan
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Inhibition of glucuronomannan hexamer on the proliferation of lung cancer through binding with immunoglobulin G, W. Jin, X. He, J. Zhu, Q. Fang, B. Wei, J. Sun, W. Zhang, Z. Zhang, F. Zhang, R. J. Linhardt, H. Wang, W. Zhong, Carbohydrate Polymers, 248, 116785, 2020.
The anti-lung cancer activity of oligosaccharides derived from glucuronomannan was investigated. The inhibition of A549 cell proliferation by glucuronomannan (Gn) and its oligomers (dimer (G2), tetramer (G4) and hexamer (G6)) were concentration dependent. In vivo activities on the A549-derived tumor xenografts showed the tumor inhibition of G2, G4 and G6 were 17 %, 40 % and 46 %, respectively. Organ coefficients in nude mice showed an increase in the kidney with G4, the brain with G6, and the spleen with G6. An advanced tandem mass tag labeled proteomics approach was performed. A significant differential expression was found in 59 out of the 4371 proteins, which involved the immune system. Surface plasmon resonance (SPR) studies revealed G6 was strongly bound to immunoglobulin G. This suggests that glucuronomannan hexamer inhibits the proliferation of lung cancer through its binding to immunoglobulin.
Carbohydrate Polymers, 248, 116785
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Polymers