Thin Layer Chromatography for the Separation and Analysis of Acidic Carbohydrates

Authors
Zhang, Zhenqing
Xiao, Zhongping
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2009-10-13
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
Terms of Use
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Full Citation
Thin Layer Chromatography for the Separation and Analysis of Acidic Carbohydrates, Z. Zhang, Z. Xiao, R.J. Linhardt, Journal of Liquid Chromatography & Related Technologies, 32, 1711–1732, 2009.
Abstract
Thin layer chromatography (TLC) is routinely used by synthetic organic chemists and natural products chemists working on a variety of types of molecules. Carbohydrate chemists and biochemists in the past largely relied on paper chromatography for rapid and inexpensive analysis. Carbohydrates are highly polar molecules and often require derivatization to be analyzed by TLC. Acidic carbohydrates pose an even more difficult challenge as they contain a formal negative charge and counterion, and can interact with commonly used stationary phases. This review describes TLC methods that have been developed for the analysis of acidic monosaccharides, disaccharides, and oligosaccharides derived from natural sources.
Description
Journal of Liquid Chromatography & Related Technologies, 32, 1711–1732
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Liquid Chromatography and Related Technologies
https://harc.rpi.edu/
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