The Road to Animal-Free Glycosaminoglycan Production: Current Efforts and Bottlenecks
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Authors
Badri, Abinaya
Williams, Asher
Linhardt, Robert J.
Koffas, Mattheos AG
Issue Date
2018-10-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Animal-extraction, despite its limitations, continues to monopolize the fast-growing glycosaminoglycan (GAG) industry. The past few years have seen an increased interest in the development of alternative GAG production methods. Chemical and chemo-enzymatic synthesis and biosynthesis from GAG producing cells, including engineered recombinant strains, are currently under investigation. Despite achieving considerable successes, these alternate approaches cannot yet meet worldwide demands for these important polysaccharides. Bottlenecks associated with achieving high-titers need to be addressed using newly developed tools. Several parameters including chassis choice, analytics, intracellular precursor synthesis, enzyme engineering and use of synthetic biology tools need to be optimized. We envision that new engineering approaches together with advances in the basic biology and chemistry of GAGs will move GAG production beyond its currently limited supply chain.
Description
Current Opinion in Biotechnology, 53, 85–92
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
The Road to Animal-Free Glycosaminoglycan Production: Current Efforts and Bottlenecks, A. Williams, A. Badri, R.J. Linhardt, M. Koffas, Current Opinion in Biotechnology, 53, 85–92, 2018.
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Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
18790429
9581669
9581669