Functionalization of electrospun nanofibers and fiber alignment enhance neural stem cell proliferation and neuronal differentiation

Amores de Sousa, Miriam C.
Rodrigues, Carlos A.V.
Ferreira, Inês A. F.
Diogo, Maria Margarida
Linhardt, Robert J.
Cabral, Joaquim M.S.
Ferreira, Frederico Castelo
Thumbnail Image
Other Contributors
Issue Date
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Terms of Use
Attribution 3.0 United States
CC BY : this license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
Functionalization of electrospun nanofibers and fiber alignment enhance neural stem cell proliferation and neuronal differentiation, M. C. Amores de Sousa, C. A. V. Rodrigues, I. A. F. Ferreira, M. Margarida Diogo, R. J. Linhardt, J. M. S. Cabral, F. Castelo Ferreira, Frontiers in Bioengineering and Biotechnology, 8, 580135, 2020.
Neural stem cells (NSCs) have the potential to generate the cells of the nervous system and, when cultured on nanofiber scaffolds, constitute a promising approach for neural tissue engineering. In this work, the impact of combining nanofiber alignment with functionalization of the electrospun poly-ε-caprolactone (PCL) nanofibers with biological adhesion motifs on the culture of an NSC line (CGR8-NS) is evaluated. A five-rank scale for fiber density was introduced, and a 4.5 level, corresponding to 70–80% fiber density, was selected for NSC in vitro culture. Aligned nanofibers directed NSC distribution and, especially in the presence of laminin (PCL-LN) and the RGD-containing peptide GRGDSP (PCL-RGD), promoted higher cell elongation, quantified by the eccentricity and axis ratio. In situ differentiation resulted in relatively higher percentage of cells expressing Tuj1 in PCL-LN, as well as significantly longer neurite development (41.1 ± 1.0 μm) than PCL-RGD (32.0 ± 1.0 μm), pristine PCL (25.1 ± 1.2 μm), or PCL-RGD randomly oriented fibers (26.5 ± 1.4 μm), suggesting that the presence of LN enhances neuronal differentiation. This study demonstrates that aligned nanofibers, functionalized with RGD, perform as well as PCL-LN fibers in terms of cell adhesion and proliferation. The presence of the full LN protein improves neuronal differentiation outcomes, which may be important for the use of this system in tissue engineering applications.
Frontiers in Bioengineering and Biotechnology, 8, 580135
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Frontiers Media SA
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Frontiers in Bioengineering and Biotechnology
A full text version is available in DSpace@RPI
CC BY — Creative Commons Attribution