A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis

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Authors
Wang, Feng
Ma, Yong
Liu, Yanhua
Cui, Zhenggang
Ying, Xiaoyan
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2017-02-11
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
A novel and industrially applicable strategy for separating and purifying the water-soluble polysaccharides from the fresh Spirulina platensis is introduced in this study. After hot water extraction, using chitosan flocculation treatment, 86.0% of the particles and 85.9% of the pigments in the crude extract were removed. The purity of polysaccharides could be increased to 94.6% and 96.1% by applying the macroporous resin ADS-7 to remove the impurities in the mode of static adsorption and in a dynamic mode of operation, respectively. The polysaccharides obtained were composed of three major components having molecular weights of 1400, 420 and 2 kDa.
Description
Separation Science and Technology, 52, 456-466
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Full Citation
A simple strategy for the separation and purification of water-soluble polysaccharides from the fresh Spirulina platensis, F. Wang, Y. Ma, Y. Liu, Z. Cui, X. Ying, F. Zhang, R. J. Linhardt, Separation Science and Technology, 52, 456-466, 2017.
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ISSN
15205754
1496395
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