Homogalacturonan from squash: Characterization and tau-binding pattern of a sulfated derivative

Zhang, Yu
Liu, Panhang
Wang, Chunyu
Zhang, Fuming
Linhardt, Robert J.
Eliezer, David
Li, Quanhong
Zhao, Jing
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Homogalacturonan from squash: Characterization and tau-binding pattern of a sulfated derivative, Y. Zhang, P. Liu, C. Wang, F. Zhang, R.J. Linhardt, D. Eliezer, Q. Li, J. Zhao, Carbohydrate Polymers, 285, 119250, 2022.
A pectic polysaccharide (WAP) was isolated from squash and identified as a homogalacturonan with a molecular mass of 83.2 kDa by GPC, monosaccharide composition analysis, FT-IR and NMR spectra. Sulfation modification of WAP was carried out and a sulfated derivative (SWAP) was obtained with a substitution degree of 1.81. The NMR spectrum indicated that the sulfation modification mainly occurred at the C-2 and C-3 positions of galacturonan residues. The binding pattern of SWAP to tau K18 protein was observed in 2D 1Hsingle bond15N HSQC spectra of tau, which resembled the tau-heparin interaction, with R2 domain as the major binding region. These results suggest that SWAP has the potential to act as a heparin mimic to inhibit the transcellular spread of tau; thus natural polysaccharide from squash may be developed into therapies for AD and related tauopathies.
Carbohydrate Polymers, 285, 119250
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Carbohydrate Polymers