Polybenzimidazole nanofibers for neural stem cell culture
Loading...
Authors
Garrudo, F.F.F.
Udangawa, R.N.
Hoffman, P.R.
Sordini, L.
Chapman, C.A.
Mikael, P.E.
Ferreira, F.A.
Silva, J.C.
Rodrigues, C.A.V.
Cabral, J.M.S.
Issue Date
2019-12-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Neurodegenerative diseases compromise the quality of life of an increasing number of people in the world's aging population. While diagnosis is possible, no effective treatments are available. Strong efforts are needed to develop new therapeutic approaches, namely, in the areas of tissue engineering and deep brain stimulation. Conductive polymers are the ideal material for these applications owing to the positive effect of conducting electricity on the neural cell's differentiation profile. This novel study assessed the biocompatibility of polybenzimidazole (PBI) as electrospun fibers and after being doped with different acids. First, doped films of PBI were used to characterize the materials' contact angle and electroconductivity. After this, fibers were electrospun and characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermogravimetric analysis. Neural stem cell (NSC) proliferation was assessed, and their growth rate and morphology on different samples were determined. Differentiation of NSCs on PBI-β-camphorsulfonic acid (CSA) fibers was also investigated, and gene expression (SOX2, NES, GFAP, and Tuj1) was assessed through immunochemistry and quantitative real-time polymerase chain reaction. All the samples tested were able to support NSC proliferation without significant changes on the cell's typical morphology. Successful differentiation of NSCs toward neural cells on PBI-CSA fibers was also achieved. This promising PBI fibrous scaffold material is envisioned to be used in neural cell engineering applications, including scaffolds and in vitro models for drug screening and electrodes.
Description
Materials Today Chemistry, 14, 100185
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Polybenzimidazole nanofibers for neural stem cell culture, F. F. F. Garrudo, R. N. Udangawa, P. R. Hoffman, L. Sordini, C. A. Chapman, P. E. Mikael, F. A. Ferreira, J. C. Silva, C. A. V. Rodrigues, J. M. S. Cabral, J. M. F. Morgado, F. C. Ferreira, R J. Linhardt, Materials Today Chemistry, 14, 100185, 2019.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
24685194