Development of flexible capacitive ultrasound transducers and the use of ultrasound for bone repair

Loading...
Thumbnail Image
Authors
Wentzell, Scott A.
Issue Date
2015-05
Type
Electronic thesis
Thesis
Language
ENG
Keywords
Biomedical engineering
Research Projects
Organizational Units
Journal Issue
Alternative Title
Abstract
After stimulation by 94.5 kHz continuous wave ultrasound for 20 minutes, significant increases in the activation of the Wnt signaling pathway and concentration of intracellular calcium were observed. Daily stimulation by ultrasound showed a trend of increased osteogenic gene expression across the phases of matrix deposition, maturation and calcification by osteoblasts. Finally, the heating of osteoblasts for stimulating osteoclastogenic responses was investigated. The application of increased temperatures of 42 and 47 degrees Celsius for 5 minutes showed significant increases in the RANKL/OPG ratio in media conditioned by osteoblasts. However, the altered RANKL/OPG ratio was not able to generate increases in osteoclastogenesis for RAW 264.7 murine macrophage cells culture in the condition media. This was possibly due to high overall osteoprotegerin expression, or unwanted inducement of M1 and M2 macrophage activation in the cell population.
Description
May 2015
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
EISSN