Synthetic Oligosaccharide Stimulates and Stabilizes Angiogenesis: Structure-Function Relationships and Potential Mechanisms

Authors
Mousa, S.A.
Feng, X.
Xie, J.
Du, Y.
Hua, Y.
He, H.
O’Connor, L.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2006-08-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Full Citation
Synthetic Oligosaccharide Stimulates and Stabilizes Angiogenesis: Structure-Function Relationships and Potential Mechanisms, S.A. Mousa, X. Feng, J. Xie, Y. Du, Y. Hua, H. He, L. O’Connor, R. J. Linhardt, The Journal of Cardiovascular Pharmacology, 48, 6-14, 2006.
Abstract
To determine the proangiogenesis effect of series of saccharides and a synthetic oligosaccharide and potential mechanisms, an in vitro 3-dimensional endothelial cell sprouting (3D-ECS) assay and the chick chorioallantoic membrane (CAM) model were used. We demonstrated that a sulfated oligosaccharide significantly promotes the endothelial capillary network initiated by vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF). Furthermore, although the capillary network initiated by VEGF and b-FGF lasts no more than 7 days, addition of a sulfated oligosaccharide significantly amplifies angiogenesis and stabilizes the capillary network of new blood vessels. In the CAM model, sulfated oligosaccharide also stimulated angiogenesis. In both the CAM and the 3D-ECS assay, structure-function studies reveal that increased saccharide chain length up to the hexa- to decasaccharide show optimal proangiogenesis efficacy. In addition, the sulfation and molecular shape (branched vs linear) of oligosaccharide are important for sustained proangiogenesis efficacy. Data indicate that chemically defined synthetic oligosaccharides can play an important role in regulation of capillary structure and stability, which may contribute to future advances in therapeutic angiogenesis. The proangiogenesis efficacy of an oligosaccharide is mediated via integrin alphavbeta3 and involves mitogen-activated protein kinase signaling mechanisms.
Description
The Journal of Cardiovascular Pharmacology, 48, 6-14
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Wolters Kluwer
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Cardiovascular Pharmacology
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI