Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR

Authors
Zhao, Jing
Liu, Xinyue
Malhotra, Anju
Li, Quanhong
Zhang, Fuming
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2017-06-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Full Citation
Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR, J. Zhao, X. Liu, A. Malnotra, Q. Li, F. Zhang, R. J. Linhardt, Analytical Biochemistry, 526, 39-42, 2017.
Abstract
A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.
Description
Analytical Biochemistry, 526, 39-42
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Elsevier
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Analytical Biochemistry
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI