Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR
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Authors
Zhao, Jing
Liu, Xinyue
Malhotra, Anju
Li, Quanhong
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2017-06-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0.98 for anti-Xa and r = 0.94 for anti-IIa). This protocol provides many advantages, significantly minimizing time, cost and the complications of chromogenic assay methods.
Description
Analytical Biochemistry, 526, 39-42
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Novel Method for Measurement of Heparin Anticoagulant Activity Using SPR, J. Zhao, X. Liu, A. Malnotra, Q. Li, F. Zhang, R. J. Linhardt, Analytical Biochemistry, 526, 39-42, 2017.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697