Using two-dimensional electrophoresis to explore the link between protein kinetic stability and tree nuts allergenicity

Authors
Rubet, Jean-Pierre
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Other Contributors
Colón, Wilfredo
Royer, Catherine Ann
Bystroff, Christopher, 1960-
Barquera, Blanca L.
Issue Date
2020-08
Keywords
Chemistry
Degree
MS
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
The results show the presence of SDS-resistant oligomers/aggregates and proteins, a direct evidence of high kinetic stability, for some tree nuts and negligible amounts for others. Overall, the results did not show a clear link between protein kinetic stability and tree nut allergenicity, perhaps because the reduction of disulfide bonds present in many allergenic proteins compromises its kinetic stability. Further studies will be needed under reducing and nonreducing conditions and using additional methods to further explore the link between protein kinetic stability and its allergenic potential.
Description
August 2020
School of Science
Department
Dept. of Chemistry and Chemical Biology
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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