Using two-dimensional electrophoresis to explore the link between protein kinetic stability and tree nuts allergenicity
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Authors
Rubet, Jean-Pierre
Issue Date
2020-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
The results show the presence of SDS-resistant oligomers/aggregates and proteins, a direct evidence of high kinetic stability, for some tree nuts and negligible amounts for others. Overall, the results did not show a clear link between protein kinetic stability and tree nut allergenicity, perhaps because the reduction of disulfide bonds present in many allergenic proteins compromises its kinetic stability. Further studies will be needed under reducing and nonreducing conditions and using additional methods to further explore the link between protein kinetic stability and its allergenic potential.
Description
August 2020
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY