Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed

Wang, Ze Ping
Shen, Jian Zhong
Linhardt, Robert J.
Jiang, Hui
Cheng, Lin Li
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Liquid to liquid extraction and liquid chromatography-tandem mass spectrometry determination of hainanmycin in feed, Z. P. Wang, J. Z. Shen, R. Linhardt, H. Jiang, L. L. Cheng, Journal of Chromatography B, 1046, 98–101, 2017.
Hainanmycin is a new veterinary polyether antibiotic and has few sensitive analytical method in present days. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) relying on multiple reaction monitoring (MRM) detection was developed for analysis of hainanmycin in animal feed. Feed samples were extracted with ethyl acetate and purified by two steps of liquid-liquid extraction (LLE) to get rid of water solvable matrix and lipids one by one. The final simple was analyzed by LC-MS/MS. The LC mobile phase was composed of 0.1% aqueous formic acid and 0.1% formic acidified acetonitrile by gradient elution. Average recoveries ranged from 74.22% to 87.85%, as determined by spiking with 2.0 (LOQ) ∼2500μgkg-1 of hainanmycin. The inter-day and intra-day coefficient of variation was 9.21% to 11.77% and 7.67% to 13.49%, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were 0.36μgkg-1 and 2.0μgkg-1, respectively.
Journal of Chromatography B, 1046, 98–101
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences