Identification, Repair and Characterization of a Benzyl Alcohol-Inducible Promoter for Recombinant Proteins Overexpression in Corynebacterium glutamicum
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Authors
Liu, Xiuxia
Zhao, Zihao
Dong, Guibin
Li, Ye
Peng, Feng
Liu, Chunli
Zhang, Fuming
Linhardt, Robert J.
Yang, Yankun
Bai, Zhonghu
Issue Date
2020-11-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Corynebacterium glutamicum is an important industrial organism for the production of a variety of biological commodities. We discovered a promoter encoded by the gene NCgl2319 in C. glutamicum, which could be induced by benzyl alcohol, could be used as an efficient tunable expression system. In initial attempts, this promoter failed to function in a recombinant expression system. This was remedied by extending the original genetic context of the promoter, generating a new version Pcat-B. The Pcat-B transcription initiation site, its critical active regions, and its effect of inducers were fully characterized resulting in tunable expression. This approach proved to be very efficient in producing a pharmaceutical protein, N-terminal pro-brain natriuretic peptide (NT-proBNP). Production of approximately 440.43 mg/L NT-proBNP was achieved with the Pcat-B expression system demonstrating its application for controllable pharmaceutical protein production in C. glutamicum.
Description
Enzyme and Microbial Technology,141, 109651
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Identification, Repair and Characterization of a Benzyl Alcohol-Inducible Promoter for Recombinant Proteins Overexpression in Corynebacterium glutamicum, X. Liu, Z. Zhao, G. Dong, Y. Li, F. Peng, C. Liu, F. Zhang, R. J. Linhardt, Y. Yang, Z. Bai, Enzyme and Microbial Technology,141, 109651, 2020.
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Journal
Volume
Issue
PubMed ID
DOI
ISSN
18790909
1410229
1410229