Sequencing the dermatan sulfate chain of decorin
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Authors
Yu, Yanlei
Duan, Jiana
Leach, Franklin E.
Toida, Toshihiko
Higashi, Kyohei
Zhang, Hong
Zhang, Fuming
Amster, I. Jonathan
Linhardt, Robert J.
Issue Date
2017-11-22
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic analysis. One of the most structurally complex classes of glycoconjugates is the proteoglycans (PGs) and their glycosaminoglycan (GAG) side chains. Previously, our laboratory solved the structure of the chondroitin sulfate chain of the bikunin PG. The current study examines the much more complex structure of the dermatan sulfate GAG chain of decorin PG. By utilizing sophisticated separation methods followed by compositional analysis, domain mapping, and tandem mass spectrometry coupled with analysis by a modified genetic algorithm approach, the structural motif for the decorin dermatan sulfate chain was determined. This represents the second example of a GAG with a prominent structural motif, suggesting that the structural variability of this class of glycoconjugates is somewhat simpler than had been expected.
Description
Journal of the American Chemical Society, 139, 16986–16995
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Sequencing the dermatan sulfate chain of decorin, Yanlei Yu, Jiana Duan, Franklin E Leach III, Toshihiko Toida, Kyohei Higashi, Hong Zhang, Fuming Zhang, I Jonathan Amster, Robert J Linhardt, Journal of the American Chemical Society, 139, 16986–16995, 2017.
Publisher
American Chemical Society (ACS)
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15205126
27863
27863