Effect of Carboxyl-Reduced Heparin on the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells
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Authors
Garg, Hari G.
Mrabat, Hicham
Yu, Lunyin
Freeman, Craig
Li, Boyangzi
Zhang, Fuming
Linhardt, Robert J.
Hales, Charles A.
Issue Date
2010-06-16
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparin (HP) inhibits the proliferation of bovine pulmonary artery smooth muscle cells (BPASMC's), among other cell types in vitro. In order to develop a potential therapeutic agent to reverse vascular remodeling, we are involved in deciphering the relationship between the native HP structure and its antiproliferative potency. We have previously reported the influence of the molecular size and the effects of various O-sulfo and N-acetyl groups of HP on growth-inhibitory activity. In this study, to understand the influence of carboxyl groups in the HP structure required for endogenous activity, a chemically modified derivative of native HP was prepared by converting the carboxyl groups of hexuronic acid residues in HP to primary hydroxyl groups. This modification procedure involves the treatment of HP with N-(3-dimethylaminopropyl)-N-ethylcarbodiimide followed by reduction with NaBH(4) to yield carboxyl-reduced heparin (CR-HP). When compared to the antiproliferative potency of native HP on cultured BPASMC's at three dose levels (1, 10, and 100 microg/mL), the CR-HP showed significantly less potency at all the doses. These results suggest that hexuronic acid residues in both major and variable sequences in HP are essential for the antiproliferative properties of native HP.
Description
Carbohydrate Research, 345, 1084–1087
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Effect of Carboxyl-Reduced Heparin on the Growth Inhibition of Bovine Pulmonary Artery Smooth Muscle Cells, H.G. Garg, H. Mrabat, L. Yu, C. Freeman, B. Li, F. Zhang, R. J. Linhardt, C. A. Hales, Carbohydrate Research, 345, 1084–1087, 2010.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
86215