Purification of non-mAb biologics : development of integrated purification processes

Authors
Goodwine, Chaz
ORCID
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Other Contributors
Cramer, Steven M.
Karande, Pankaj
Bystroff, Christopher, 1960-
Gross, Richard
Issue Date
2018-08
Keywords
Chemical engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
The first strategy employed for purification process development consisted of identifying affinity peptides for use as chromatographic ligands to selectively capture desired product directly from cell culture fluid. In this strategy, epitope mapping of known target binding partners was used to generate an initial library of peptides. Further, phage display screening with negative selection was used to identify additional peptides to supplement the library of initial peptide candidates. Using immobilized peptide microarrays, the library of candidates was screened to evaluate the affinity and selectivity of the peptides towards granulocyte colony-stimulating factor. Lead peptide candidates identified by this screening method were further evaluated in fluorescence polarization assays to determine their ability to be utilized as affinity ligands.
Description
August 2018
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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