Determination of the Composition of the Oligosaccharide Phosphate Fraction of Pichia holstii NRRL Y-2448 Phosphomannan by Capillary Electrophoresis and HPLC
No Thumbnail Available
Authors
Ferro, Vito
Li, Caiping
Fewings, Kym
Palermo, Maria C.
Linhardt, Robert J.
Toida, Toshihiko
Issue Date
2002-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The promising new anticancer agent, PI-88, is prepared by the sulfonation of the oligosaccharide phosphate fraction of the extracellular phosphomannan produced by the yeast Pichia (Hansenula) holstii NRRL Y-2448. The composition of the oligosaccharide phosphate fraction was determined by capillary electrophoresis (CE) with indirect UV detection using 6 mM potassium sorbate at pH 10.3 as the background electrolyte. Further confirmation of the composition was obtained by HPLC analysis of a sample dephosphorylated by treatment with alkaline phosphatase. The structure of the hexasaccharide component has been determined by isolation and NMR spectroscopic analysis of its dephosphorylated derivative. Additionally, the structure of a second, previously undetected tetrasaccharide component (a hexosamine) has been determined by isolation and NMR spectroscopic analysis of the acetate of its dephosphorylated derivative. It is demonstrated that CE is an ideal method for the quality control of the oligosaccharide phosphate fraction for use in the production of PI-88.
Description
Carbohydrate Research, 337, 139-146
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Determination of the Composition of the Oligosaccharide Phosphate Fraction of Pichia holstii NRRL Y-2448 Phosphomannan by Capillary Electrophoresis and HPLC, V. Ferro, C. Li, K. Fewings, M.C. Palermo, D. Podger, R.J. Linhardt, T. Toida, Carbohydrate Research, 337, 139-146, 2002.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
86215