Direct Measurement of the Interaction of Glycosaminoglycans and a Heparin Decasaccharide with Malaria Circumsporozoite Protein
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Authors
Rathore, D.
McCutchan, T.F.
Hernaiz, M.J.
LeBrun, L.A.
Lang, S.C.
Linhardt, Robert J.
Issue Date
2001-09-25
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC). Heparin was the best binder among the glycosaminoglycans tested and bound to CS protein with nanomolar affinity. Using purified and structurally defined small heparin oligosaccharides, we identified a decasaccharide to be the minimum sized CS protein-binding sequence. In an indirect competition assay, this decasaccharide blocked the CS protein interaction with HepG2 cells with an ID50 of less than 60 nM. The decasaccharide has a structure commonly found in hepatic heparan sulfate, and the same sequence has recently been shown to bind specifically to apolipoprotein E. Examination of porcine liver heparan sulfate in this indirect competition assay showed that it and heparin were the only glycosaminoglycans that could effectively block CS protein interaction with HepG2 cells in culture. These data support the hypothesis that the invasion of liver cells by the parasite shares a common mechanism with the hepatic uptake of lipoprotein remnants from the blood.
Description
Biochemistry, 40, 11518-11524
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Direct Measurement of the Interaction of Glycosaminoglycans and a Heparin Decasaccharide with Malaria Circumsporozoite Protein, D. Rathore, T. F. McCutchan, M. J. Hernaiz, L.A. LeBrun, S.C. Lang, R.J. Linhardt, Biochemistry, 40, 11518-11524, 2001.
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Volume
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PubMed ID
DOI
ISSN
62960