Synthesis of heparin immobilized-magnetically addressable cellulose nanofibers for biomedical applications
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Authors
Hou, Lijuan
Udangawa, W. M.Ranodhi N.
Pochiraju, Anirudh
Dong, Wenjun
Zheng, Yingying
Linhardt, Robert J.
Simmons, Trevor J.
Issue Date
2016-11-14
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Magnetically responsive heparin-immobilized cellulose nanofiber composites were synthesized by wet-wet electrospinning from a nonvolatile, room-temperature ionic liquid (RTIL), 1-methyl-3-methylimidazolium acetate ([EMIM][Ac]), into an aqueous coagulation bath. Superparamagnetic magnetite (Fe3O4) nanoparticles were incorporated into the fibers to enable the manipulation of both dry and wet nanofiber membranes with an external magnetic field. Three synthetic routes were developed to prepare three distinct types of nanocomposite fibers: cellulose-Fe3O4–heparin monofilament fibers, cellulose-Fe3O4–heparin core–shell fibers with heparin covalently immobilized on the fiber surface, and cellulose -Fe3O4 core–shell fibers with heparin physically immobilized on the fiber surface. These nanocomposite fibers were characterized by electron microscopy to confirm their coaxial structure and the fiber dimensions (fiber diameter 0.2–2.0 μm with 0.1–1.1 μm core diameter). Thermogravimetric analysis, liquid chromatography–mass spectrometry, Fourier transform infrared and X-ray diffraction spectroscopy provided detailed compositional analysis for these nanocomposite fibers, confirming the presence of each component and the surface accessibility of the heparin. The anticoagulant activity of immobilized heparin on the nanocomposite fiber surfaces was evaluated and confirmed by antifactor Xa and antifactor IIa assays.
Description
ACS Biomaterials Science & Engineering, 2, 1905−1913
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Synthesis of heparin immobilized-magnetically addressable cellulose nanofibers for biomedical applications L. Hou, W.M. R. Udangawa, A. Pochiraju, W. Dong, Y. Zheng, R. J. Linhardt, T. Simmons, ACS Biomaterials Science & Engineering, 2, 1905−1913, 2016.
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ISSN
23739878