Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa
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Authors
Zhang, Anqiang
Deng, Jiaying
Yu, Shuying
Zhang, Fuming
Linhardt, Robert J.
Sun, Peilong
Issue Date
2018-08-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04×106Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (1H NMR and 13C NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be β‑d‑Glcp‑(1→[4)‑α‑d‑Glcp‑(1→4)‑α‑d‑Glcp‑(1]m→4)‑α‑d‑Glcp.
Description
International Journal of Biological Macromolecules, 115, 221–226
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa. A. Zhang, J. Deng, S. Yu, F. Zhang, R. J. Linhardt, P. Sun, International Journal of Biological Macromolecules, 115, 221–226, 2018.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
18790003
1418130
1418130