Cell-based microscale isolation of glycoaminoglycans for glycomics study

Zhao, Xue
Yang, Bo
Datta, Payel
Gasmili, Leyla
Zhang, Fuming
Linhardt, Robert J.
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Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Cell-based microscale isolation of glycoaminoglycans for glycomics study, X. Zhao, B. Yang, P. Dutta, L. Gasmili, F. Zhang, R. J. Linhardt, Journal of Carbohydrate Chemistry, 31, 420-435, 2012.
Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, moderately sulfated, and nonsulfated glycosaminoglycans (GAGs) was developed using Chinese hamster ovary (CHO) cells. This microscale isolation relies on a mini-strong anion exchange spin column eluted stepwise with different concentrations of sodium chloride solution. Hyaluronic acid, chondroitin sulfate, and heparin were used to optimize the isolation of the endogenous glycosaminoglycans in CHO cells. This method can also be used to determine the presence of nonsulfated GAGs including heparosan, hyaluronic acid, and nonsulfated chondroitin.
Journal of Carbohydrate Chemistry, 31, 420-435
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The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Taylor and Francis
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Journal of Carbohydrate Chemistry
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