Cell-based microscale isolation of glycoaminoglycans for glycomics study
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Authors
Zhao, Xue
Yang, Bo
Datta, Payel
Gasmili, Leyla
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2012-07-09
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Glycomics research requires the isolation of glycans from cells for structural characterization and functional studies of the glycans. A method for cell-based microscale isolation and quantification of highly sulfated, moderately sulfated, and nonsulfated glycosaminoglycans (GAGs) was developed using Chinese hamster ovary (CHO) cells. This microscale isolation relies on a mini-strong anion exchange spin column eluted stepwise with different concentrations of sodium chloride solution. Hyaluronic acid, chondroitin sulfate, and heparin were used to optimize the isolation of the endogenous glycosaminoglycans in CHO cells. This method can also be used to determine the presence of nonsulfated GAGs including heparosan, hyaluronic acid, and nonsulfated chondroitin.
Description
Journal of Carbohydrate Chemistry, 31, 420-435
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Cell-based microscale isolation of glycoaminoglycans for glycomics study, X. Zhao, B. Yang, P. Dutta, L. Gasmili, F. Zhang, R. J. Linhardt, Journal of Carbohydrate Chemistry, 31, 420-435, 2012.
Publisher
Taylor and Francis
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15322327
7328303
7328303