Study of Defined Oligosaccharide Substrates of Heparin and Heparan Monosulfate Lyases

Rice, Kevin G.; Linhardt, Robert J.

Study of Defined Oligosaccharide Substrates of Heparin and Heparan Monosulfate Lyases

Authors

Rice, Kevin G.

Linhardt, Robert J.

Issue Date

1989-07-15

Type

Article

Language

ENG

Keywords

Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering

Abstract

The rapid preparation of multimilligram quantities of five heparin-derived oligosaccharides (1-5) is described. These oligosaccharides are the final products obtained from the action of heparin lyase (heparinase, E.C. 4.2.2.7) at its primary sites in the heparin polymer. Five oligosaccharides comprise from 75-85 wt% of commercial porcine mucosal heparins and are recovered in good yield and high purity. Four of these five oligosaccharides were further acted upon at much lower rates by prolonged treatment with heparin lyase or heparan monosulfate lyase (heparitinase, E.C. 4.2.2.8), revealing the subspecificities of these enzymes. These oligosaccharides were used as defined substrates for heparin lyase and heparan monosulfate lyase and their kinetic constants were obtained. Potential applications for these oligosaccharides include their use as defined substrates for purification of heparin monosulfate lyases, and for establishing the catalytic purity of enzyme preparations.

Description

Carbohydrate Research, 190, 219-233
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.

Full Citation

Study of Defined Oligosaccharide Substrates of Heparin and Heparan Monosulfate Lyases, K.G. Rice and R.J. Linhardt, Carbohydrate Research, 190, 219-233 (1989).

URI

https://doi.org/10.1016/0008-6215(89)84127-8
https://hdl.handle.net/20.500.13015/6015

ISSN

86215

Collections

Linhardt Research Labs Papers

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