Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides

Authors
Miller, Michelle C.
Cai, Chao
Wichapong, Kanin
Bhaduri, Sayantan
Pohl, Nicola L.B.
Linhardt, Robert J.
Gabius, Hans Joachim
Mayo, Kevin H.
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2020-12-01
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Attribution 3.0 United States
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Full Citation
Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides, M. C. Miller, C. Cai, K. Wichapong, S. Bhaduri, N. L.B. Pohl, R. J. Linhardt, H. -J. Gabius, K. H. Mayo, Science Reports, 10, 15708, 2020.
Abstract
Glycosaminoglycan chains of keratan sulfate proteoglycans appear to be physiologically significant by pairing with tissue lectins. Here, we used NMR spectroscopy and molecular dynamics (MD) simulations to characterize interactions of corneal keratan sulfate (KS), its desulfated form, as well as di-, tetra- (N-acetyllactosamine and lacto-N-tetraose) and octasaccharides with adhesion/growth-regulatory galectins, in particular galectin-3 (Gal-3). The KS contact region involves the lectin canonical binding site, with estimated KD values in the low µM range and stoichiometry of ~ 8 to ~ 20 galectin molecules binding per polysaccharide chain. Compared to Gal-3, the affinity to Gal-7 is relatively low, signaling preferences among galectins. The importance of the sulfate groups was delineated by using desulfated analogs that exhibit relatively reduced affinity. Binding studies with two related di- and tetrasaccharides revealed a similar decrease that underscores affinity enhancement by repetitive arrangement of disaccharide units. MD-based binding energies of KS oligosaccharide-loaded galectins support experimental data on Gal-3 and -7, and extend the scope of KS binding to Gal-1 and -9N. Overall, our results provide strong incentive to further probe the relevance of molecular recognition of KS by galectins in terms of physiological processes in situ, e.g. maintaining integrity of mucosal barriers, intermolecular (lattice-like) gluing within the extracellular meshwork or synaptogenesis.
Description
Science Reports, 10, 15708
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Nature
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Scientific Reports
https://harc.rpi.edu/
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A full text version is available in DSpace@RPI
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