Gradient Polyacrylamide Gel Electrophoresis for Determination of the Molecular Weight of Heparin and Low Molecular Weight Heparin Derivatives
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Authors
Edens, R.E.
Al-Hakim, A.
Weiler, J.M.
Rethwisch, D.G.
Fareed, J.
Linhardt, Robert J.
Issue Date
1992-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The M(r) values of pharmaceutical heparins and low-molecular-weight (LMW) heparin derivatives were examined as part of a collaborative study to develop methods for their characterization. Standard methods of M(r) determination rely on gel permeation high-performance liquid chromatography (HPLC). We report the use of gradient polyacrylamide gel electrophoresis (PAGE) to determine the M(r) values of pharmaceutical heparins and LMW heparin derivatives. This approach offers certain advantages over the HPLC method. Gradient PAGE analysis was performed in parallel, on multiple samples, with the same standard curve. HPLC was performed serially. Gradient PAGE gave higher resolution than HPLC, and thus, a mixture of easily obtained standards was used in place of individual standards for the construction of a standard curve. Heparin and various LMW heparin samples were analyzed by both gradient PAGE and conventional gel permeation HPLC methods. The number-average M(r), weight-average M(r), and polydispersity were examined by both techniques and found to be similar. This study demonstrates that gradient PAGE analysis is a sensitive method for the determination of the M(r) values of heparin and LMW heparin.
Description
Journal of Pharmaceutical Sciences, 81, 823-827
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Gradient Polyacrylamide Gel Electrophoresis for Determination of the Molecular Weight of Heparin and Low Molecular Weight Heparin Derivatives, R.E. Edens, A. Al-Hakim, J.M. Weiler, D.G. Rethwisch, J. Fareed, R.J. Linhardt, Journal of Pharmaceutical Sciences, 81, 823-827 (1992).
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Journal
Volume
Issue
PubMed ID
DOI
ISSN
15206017
223549
223549