Analysis of total human urinary glycosaminoglycan disaccharides by liquid chromatography-tandem mass spectrometry
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Authors
Sun, Xiaojun
Li, Lingyun
Overdier, Katherine H.
Ammons, Lee Anne
Douglas, Ivor S.
Burlew, Clay Cothren
Zhang, Fuming
Schmidt, Eric P.
Chi, Lianli
Linhardt, Robert J.
Issue Date
2015-06-16
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The determination of complex analytes, present at low concentrations, in biological fluids poses a difficult challenge. This study relies on an optimized method of recovery, enzymatic treatment, and disaccharide analysis by liquid chromatography–tandem mass spectrometry to rapidly determine low concentrations of glycosaminoglycans in human urine. The approach utilizes multiple reaction monitoring (MRM) of glycosaminoglycan disaccharides obtained from treating urine samples with recombinant heparin lyases and chondroitin lyase. This rapid and sensitive method allows the analysis of glycosaminoglycan content and disaccharide composition in urine samples having concentrations 10- to 100-fold lower than those typically analyzed from patients with metabolic diseases, such as mucopolysaccharidosis. The current method facilitates the analysis low (ng/mL) levels of urinary glycosaminoglycans present in healthy individuals and in patients with pathological conditions, such as inflammation and cancers, that can subtly alter glycosaminoglycan content and composition.
Description
Analytical Chemistry, 87, 6220−6227
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Analysis of total human urinary glycosaminoglycan disaccharides by liquid chromatography-tandem mass spectrometry, X. Sun, L. Li, K. H. Overdier, L. A. Ammons, I. S. Douglas, C. C. Burlew, F. Zhang, E.P. Schmidt, L. Chi, R. J. Linhardt, Analytical Chemistry, 87, 6220−6227, 2015.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
15206882
32700
32700