Rational design for improving production biological activity and identification of natural products
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Authors
Bhan, Namita J.
Issue Date
2014-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical engineering
Alternative Title
Abstract
In this work, we employed metabolic engineering and systems biology to improve the yields of plant polyketide resveratrol in E. coli by redirecting carbon flux into the heterologous pathway. Further, we attempted to generate novel polyketides by structure-based engineering of stilbene synthase (STS), a type III plant polyketide synthase. Finally, we diversified the resveratrol monomer into two dimers (resveratrol-trans-dihydrodimer and pallidol) via in vitro oligomerization reaction. Resveratrol-trans-dihydrodimer was shown to be an active antimicrobial, having significantly higher potency than the monomer. Apart from that, we also attempted to employ metabolomics approach to characterize the secondary metabolites produced by a strain of marine bacteria, Pseudoalteromonas ATCC 29581.
Description
August 2014
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY