Rational design for improving production biological activity and identification of natural products

Authors
Bhan, Namita J.
ORCID
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Other Contributors
Koffas, Mattheos A. G.
Linhardt, Robert J.
Kane, Ravi S.
Barquera, Blanca L.
Issue Date
2014-08
Keywords
Chemical engineering
Degree
PhD
Terms of Use
This electronic version is a licensed copy owned by Rensselaer Polytechnic Institute, Troy, NY. Copyright of original work retained by author.
Full Citation
Abstract
In this work, we employed metabolic engineering and systems biology to improve the yields of plant polyketide resveratrol in E. coli by redirecting carbon flux into the heterologous pathway. Further, we attempted to generate novel polyketides by structure-based engineering of stilbene synthase (STS), a type III plant polyketide synthase. Finally, we diversified the resveratrol monomer into two dimers (resveratrol-trans-dihydrodimer and pallidol) via in vitro oligomerization reaction. Resveratrol-trans-dihydrodimer was shown to be an active antimicrobial, having significantly higher potency than the monomer. Apart from that, we also attempted to employ metabolomics approach to characterize the secondary metabolites produced by a strain of marine bacteria, Pseudoalteromonas ATCC 29581.
Description
August 2014
School of Engineering
Department
Dept. of Chemical and Biological Engineering
Publisher
Rensselaer Polytechnic Institute, Troy, NY
Relationships
Rensselaer Theses and Dissertations Online Collection
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