Measurement of the Antithrombin III Binding Site in Low Molecular Weight Heparins Using 13C NMR and Capillary Electrophoresis
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Authors
Desai, Umesh R.
Hoppensteadt, Debra
Fareed, Jawed
Linhardt, Robert J.
Issue Date
1995-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Low molecular weight heparins, prepared from the controlled chemical or enzymatic depolymerization of the heparin polysaccharide, are currently replacing heparin as the clinical anticoagulant/antithrombotic agent of choice. A principal mechanism of action of these agents is through the binding of antithrombin III, a plasma serine protease inhibitor, to specific pentasaccharide sequences in these polysaccharides. The content of antithrombin III-binding pentasaccharide sequences within low molecular weight heparins vary, making these drugs bio-inequivalent. Currently, the only way to examine the content of these pentasaccharide sequences within a low molecular weight heparin preparation relies on bioassay. This study examined both 13C NMR spectroscopy and oligosaccharide analysis by capillary electrophoresis as alternative measures of the content of antithrombin III binding sites within a variety of low molecular weight heparins. The number of antithrombin III binding sites per chain, measured by 13C NMR spectroscopy, correlated with the antithrombin III mediated anti-factor Xa activity of various low molecular weight heparins.
Description
Pharmaceutical Science, 1, 349-353
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Measurement of the Antithrombin III Binding Site in Low Molecular Weight Heparins Using 13C NMR and Capillary Electrophoresis, U.R. Desai, D. Hoppensteadt, J. Fareed, R.J. Linhardt, Pharmaceutical Science, 1, 349-353, 1995.
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
20427158
14608081
14608081