Predictive scale-up of ion exchange chromatography using high throughput miniature columns

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Authors
Keller, William R.
Issue Date
2015-12
Type
Electronic thesis
Thesis
Language
ENG
Keywords
Chemical engineering
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Abstract
A detailed comparison was carried out using both experimental data and column simulations for miniature and traditional benchtop columns. Appropriate translations to account for extracolumn system volume differences were developed and shown to result in comparability. A parameter estimation protocol was then implemented to obtain linear steric mass-action (SMA) isotherm and lumped mass transport parameters from gradient experiments in both systems. The parameters estimated from the miniature columns were successfully employed to simulate the benchtop experimental data.
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December 2015
School of Engineering
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Rensselaer Polytechnic Institute, Troy, NY
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