Electron Detachment Dissociation of Glycosaminoglycan Tetrasaccharides
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Authors
Wolff, J.J.
Chi, L.
Linhardt, Robert J.
Amster, I.J.
Issue Date
2007
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
The first application of electron detachment dissociation (EDD) to carbohydrates is presented. The structural characterization of glycosaminoglycan (GAG) oligosaccharides by mass spectrometry is a longstanding problem because of the lability of these acidic, polysulfated carbohydrates. Doubly-charged negative ions of four GAG tetrasaccharides are examined by EDD, collisionally activated dissociation (CAD), and infrared multiphoton dissociation (IRMPD). EDD is found to produce information-rich mass spectra with both cross ring and glycosidic cleavage product ions. In contrast, most of the product ions produced by CAD and IRMPD result from glycosidic cleavage. EDD shows great potential as a tool for locating the sites of sulfation and other modifications in glycosaminoglycan oligosaccharides.
Description
Journal of the American Society of Mass Spectrometry, 18, 234-244
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Electron Detachment Dissociation of Glycosaminoglycan Tetrasaccharides, J. J. Wolff, L. Chi, R. J. Linhardt, I. J. Amster, Journal of the American Society of Mass Spectrometry, 18, 234-244, 2007.
Publisher
American Chemical Society (ACS)