[[The]] negative arm of the circadian clock imparts post-transcriptional regulation in neurospora crassa
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Authors
Mosier, Alexander Eric
Issue Date
2021-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Biology
Alternative Title
Abstract
We examined the intrinsically disordered nature of FRQ to establish conformational variability over the circadian day and found distinct temporal difference in protease susceptibility Next tested for changes in the FRQ interactome and found that FRQ interactors were variable and involved in diverse biological processes, further supporting the “hub” theory of regulation. Next, we analyzed these interactors for levels of protein disorder and found proteins with higher levels of disorder to more commonly bind at specific times of the day, leading to a potential regulatory pathway based on protein stabilization.. Finally, we examined one FRQ binding partner, CDC28, for post-transcriptional regulation and found that FRQ is both required for CDC28 phosphorylation and that there is circadian rhythmicity to this post-translational modification.
Description
May 2021
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY